Fig. 8.
![Fig. 8. Metabolism of [3H]C6-Cer in HUVECs. (A) HUVECs were incubated with [3H]C6-Cer for various durations. Lipids were then extracted from cells and analyzed for [3H]C6-Cer metabolism. Locations of standard lipids are indicated on the left. (B) HUVECs were incubated with [3H]C6-Cer for 1 minute and then challenged without (C) or with 1 U/mL of thrombin (Thr), 1 μmol/L TPA, 10 μmol/L LPA, 1 μmol/L angiotensin II (A II), 1 μmol/L AVP, or 1 μmol/L PAF for 20 minutes in the presence of a 1% FCS medium.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/12/10.1182_blood.v93.12.4293/4/blod41226008w.jpeg?Expires=1726821337&Signature=ovi4pzDpMK45NOfh5SLw-URiVVKSI2D~b52bZj0-kFkLrohU9pBEvlMgxmCtkDExXuHmvr3MkIK0RWZv5rZmRtfHOvhomb5YNmrDE~Z8IZzOdHSQ75TtCH-bkBZdbHgSqLZsQVOxledoD1QoazfBjxlO03e4c~V4d-~1lJ7-rzks7RQxxr1znUfkMLR6DJZZDd9tTyYwMadO~dSm1NkKjJXkqCo2mXqhmjet8oP0G9j55Gx~EWrTgmtECOaUous-q0GqvJq6lA5tr3UMlBhTJuIe4rfUCSgj6GiZaHnpcmXq1whWZldXs6~nHoi0-fKSIrzOBnagE1AJsKe-TazPNw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Metabolism of [3H]C6-Cer in HUVECs. (A) HUVECs were incubated with [3H]C6-Cer for various durations. Lipids were then extracted from cells and analyzed for [3H]C6-Cer metabolism. Locations of standard lipids are indicated on the left. (B) HUVECs were incubated with [3H]C6-Cer for 1 minute and then challenged without (C) or with 1 U/mL of thrombin (Thr), 1 μmol/L TPA, 10 μmol/L LPA, 1 μmol/L angiotensin II (A II), 1 μmol/L AVP, or 1 μmol/L PAF for 20 minutes in the presence of a 1% FCS medium.
