Fig. 7.
![Fig. 7. Effects of various agents on [3H]Sph metabolism in HUVECs. HUVECs were incubated with [3H]Sph for 1 minute and then challenged without (C) or with 100 U/mL of IL-1β (IL-1), 1 U/mL of thrombin (Thr), or 1 μmol/L TPA for 20 minutes in the presence of a 1% FCS medium. Lipids were extracted from cells or media and analyzed for [3H]Sph metabolism. Locations of standard lipids are indicated on the left.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/12/10.1182_blood.v93.12.4293/4/blod41226007w.jpeg?Expires=1726821338&Signature=JV6TTQOS-yJ9lk8u6TYOzAlh24ijNFoJqfHuQGxck0BNTN4jlHKX6HGaFXi~vJ8c81hbGww-VtnNETZyMQ8KB8~D397brC5V-kLKs4svEZ4OsNvPC12p0g6cc5tnSp2GkDdP9gYH0euHMsc-nTQEi7RK0GZ1RwB84wVDu8teLDF26Q9h7F5GNX3RkoYaMDuCXDR-O~E2fHiHa3gMrhMyVGfYX~DNjUVRNDozK42PRWGs7E2cWn1vY2Ulxbn-vcOFts0GIpWj0alGBeLruAedrtUQDkFhQQ3XPLZErOnm18fn56F5bF9X7k2VYIvnNjuLN2adPfZvoGWB604Iectg8w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of various agents on [3H]Sph metabolism in HUVECs. HUVECs were incubated with [3H]Sph for 1 minute and then challenged without (C) or with 100 U/mL of IL-1β (IL-1), 1 U/mL of thrombin (Thr), or 1 μmol/L TPA for 20 minutes in the presence of a 1% FCS medium. Lipids were extracted from cells or media and analyzed for [3H]Sph metabolism. Locations of standard lipids are indicated on the left.
