Analysis of clonotype specificity of cultured PBMC from patient 2. The lanes are labeled as described in Fig 5, except that only 1 plasmid control is included. (A) Spectratype gel. The actin control amplification is shown as a separate subpanel. (B) Hybridization of the DNA from the gel shown in A with a probe for clonotype 2. (C) Rehybridization of the membrane with a TCR β constant region probe after stripping of the previous probe.