T-cell receptor spectratype analysis (multiplex PCR) of 26 BV families using noncultured PBMC from a normal subject (A) and from patients 1 and 2 (B and C). Primers specific for β-actin were included in each reaction mixture as a check on fidelity. Bands in each group differ from each other by 3 bp in length because only T cells with productive (in phase) VDJ rearrangements are found in the peripheral blood.