Fig. 6.
![Fig. 6. Temperature dependence of the [Ca2+]i signal induced by exogenous arachidonic acid. The [Ca2+]i transient in a stirred suspension of human platelets (50,000 cells/μL) was monitored with Fura-PE3. The platelets were stimulated at 20°C (A), 30°C (B), or 37°C (C) by 20 μmol/L arachidonic acid (solid traces) in the presence of extracellular calcium (1 mmol/L CaCl2). Data obtained in parallel control studies with 0.2% (wt/vol) dimethylsulfoxide (vehicle for arachidonic acid; dotted traces) are also displayed. Observations at 25°C (not shown) were indistinguishable from those at 20°C. The results illustrated are from a typical one of seven such studies.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/1/10.1182_blood.v94.1.199.413k14_199_207/6/blod41314006x.jpeg?Expires=1722427310&Signature=lMoDJcUKY8QmoyVUFkkWR5EYapbeGZXtLtEntzbuQDYrwpOBqLH1JwUf2-ooPjiWvieJbM6gTvY3mFxfiRE6ce6ohbTe~H5pu9JXhIO4jFkvqEuBZ7koW9YOkUB0hbyY7~k4bDLiikv5~ICPRMvyOhGOF~AyEdFtIp9njjLz1zxUmdW8TADTQqOOdquZYClCzHI7OAe8KhtbGlmVC0rt70DlMRwvyGRrvQPL~0tNXyYoJXBWJ~ySOGV0DHIOmM5cpT3ypeDk~zCdi2M0baJo7FqRwenBKgMO-3KKtlgwiy3FLGb1ovNM0md7pg~mxOWWmL-xwantCkP8WM3oJ1ADrg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Temperature dependence of the [Ca2+]i signal induced by exogenous arachidonic acid. The [Ca2+]i transient in a stirred suspension of human platelets (50,000 cells/μL) was monitored with Fura-PE3. The platelets were stimulated at 20°C (A), 30°C (B), or 37°C (C) by 20 μmol/L arachidonic acid (solid traces) in the presence of extracellular calcium (1 mmol/L CaCl2). Data obtained in parallel control studies with 0.2% (wt/vol) dimethylsulfoxide (vehicle for arachidonic acid; dotted traces) are also displayed. Observations at 25°C (not shown) were indistinguishable from those at 20°C. The results illustrated are from a typical one of seven such studies.
