TEL-Jak2 JH1 transforms Ba/F3 cells to factor-independence. Ba/F3 cells (5 × 10⁴ cells) expressing various TEL-Jak2 constructs were incubated in the presence or absence of 100 pg/mL IL-3 for 4 days. Viable cells were enumerated by Trypan blue exclusion. (□) Ba/F3, no IL-3; (▪) Ba/F3, plus IL-3; (○) Ba/F3-HA-TEL-Jak2 JH1 subclone 36, no IL-3; (•) Ba/F3-HA-TEL-Jak2 JH1 subclone 36, plus IL-3; (▵) Ba/F3-HA-TEL-Jak2 JH2 subclone 4, no IL-3; (◊) Ba/F3-HA-TEL-KI-Jak2 subclone 11, no IL-3. The IL-3–dependent growth of Ba/F3-TEL-Jak2 JH2 and Ba/F3-TEL-KI-Jak2 JH1 was exactly similar to that of Ba/F3 cells and has been omitted for clarity.