Fig. 3.
![Fig. 3. In vitro transcription/translation analysis. Protein production from the two forms of the GP Ib cDNA was evaluated in a cell-free transcription/translation system using [35S]methionine as the radiolabel for the newly synthesized protein. The band designated GP Ib is of the molecular weight expected for the polypeptide that has not been posttranslationally modified (∼70 kD). The experiment was performed six times; a representative autoradiogram is shown. The autoradiogram was obtained by phosphorimaging; the band densities are expressed in arbitrary units. Substantially more protein was synthesized from the −5C plasmid.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/1/10.1182_blood.v94.1.186.413k19_186_191/6/blod41319003w.jpeg?Expires=1726821191&Signature=X6TsxISEgx1BfM~W5utscgou60xvQw5HsV0c-jJd7XIiZr-nt0mbjOzn55Df5ymiPO3efDTcsakozqdFIG-TfCfFvZccOekVtgdVJTvINpHh6UYjhThQzmyRHmd6PoAfJ3ZCYQQyXk4YeX~y-r8ZD1Pes3GIJBwDOGaZ4moDpVqdrqBfxXhzPNwY8wiMbzmqnysksRFvGCLkt6dj8XiJve-81kvH-UusL6UgeV9VnGQkljOSNN1dgZNsbyGvqRqKuYlwxKUGqwZ0drINUwRFr518ow2IpCmVqtwYcg-rGvIBwOZQ52UH~KB5iUQp6mGNlt3WtexY7lZNo-t7oZJKrQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
In vitro transcription/translation analysis. Protein production from the two forms of the GP Ib cDNA was evaluated in a cell-free transcription/translation system using [35S]methionine as the radiolabel for the newly synthesized protein. The band designated GP Ib is of the molecular weight expected for the polypeptide that has not been posttranslationally modified (∼70 kD). The experiment was performed six times; a representative autoradiogram is shown. The autoradiogram was obtained by phosphorimaging; the band densities are expressed in arbitrary units. Substantially more protein was synthesized from the −5C plasmid.
