Fig. 3.
Chimerism of representative irradiated recipient mice transplanted with FancC −/− or FancC +/+ BM cells 6 months after transplantation. Representative histograms of peripheral blood cells obtained from recipient mice transplanted with 500,000 low-density competitor cells expressing the CD45.1 antigen and 5,000 Sca1+lin−/dimFancC −/−cells (left panel) or 5,000 Sca1+lin−/dimFancC +/+ cells (right panel), which express the CD45.2 antigen. Nucleated cells from aliquots of each specimen were stained with antibodies to CD45.1 (
), CD45.2 (▪), and the isotype control (□). The contribution of FancC −/− and FancC +/+cells to the chimerism is indicated in the upper righthand corner of each histogram.