Fig. 3.
![Fig. 3. p21 and p27 expression in cells of colonies. Cells of colonies on day 15 of the culture generated from human cord blood CD34+ cells were immunostained using anti-p21 antibody, 6B6 (A through E), and anti-p27 antibody, F-8 (F through J). Immunostaining was performed using the APAAP method as described in Materials and Methods. Nuclei were counterstained with hematoxylin. Positive cells showed red nuclear staining. (A and F) megakaryocytes, (B and G) erythroblasts, (C and H) macrophages, (D and I) granulocytes, and (E and J) MEG-01s cells harvested 48 hours after the addition of 10 nmol/L TPA (positive control; original magnifications: [A] and [F], ×200; [B] through [E] and [G] through [J], ×50).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/12/10.1182_blood.v93.12.4167/4/blod412tan03z.jpeg?Expires=1722653669&Signature=rpWu6b-HJGIsm7nHI5s2eZG43T3EcO8UigC4A0AkgcMPJHR0KjBOeyHz8~SveqbLha6xCRKoU6ZhlLe25T3Eo5G3HehuOwIpCcT~C1yr58w0gdG6Qz0fav0zgyTpp359v39x6ZEPHvh386g9dzDhP3ZJEMHAzP4fL56myFjSEQw1ERvwh3SGznv41UoEsdhCxXX7eHUwFyTIEp9w6NqXp-4HEXJZfVK8oN5h-MGYvTgN0ZHgtrEfTYYw~gANO8HZh9TJZneu0rph3rnOg1nR~UMoTuJoWlhB8sxCrlKbYkFXuU1rRqgf8guRusz7E7WuJ4SYAvWrfjEgug-yR3cRcw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
p21 and p27 expression in cells of colonies. Cells of colonies on day 15 of the culture generated from human cord blood CD34+ cells were immunostained using anti-p21 antibody, 6B6 (A through E), and anti-p27 antibody, F-8 (F through J). Immunostaining was performed using the APAAP method as described in Materials and Methods. Nuclei were counterstained with hematoxylin. Positive cells showed red nuclear staining. (A and F) megakaryocytes, (B and G) erythroblasts, (C and H) macrophages, (D and I) granulocytes, and (E and J) MEG-01s cells harvested 48 hours after the addition of 10 nmol/L TPA (positive control; original magnifications: [A] and [F], ×200; [B] through [E] and [G] through [J], ×50).
