![Fig. 2. The role of Syk- and Src-family kinases in collagen- and CRP-mediated calcium increases in mouse megakaryocytes. Single mouse megakaryocytes from Balb/c bone marrow were microinjected with FURA-2, and the changes in [Ca2+]i were determined in response to (A) 100 μg/mL collagen or (B) 2 μg/mL CRP. The response to collagen (100 μg/mL) was then determined (C) after comicroinjection of a recombinant fusion protein containing the tandem SH2 domains of syk; (D) in syk-deficient megakaryocytes; (E) in the presence of the Src-family kinase inhibitor, PP1 (10 μmol/L for 3 minutes); or (F) in fyn-deficient megakaryocytes. Each trace is representative of 4 to 10 cells from a minimum of three different mice. In each case, [Ca2+]e was fixed at 200 μmol/L.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/11/10.1182_blood.v93.11.3847/4/blod41117002x.jpeg?Expires=1722761922&Signature=FlsWmnsQ1mRoE~IHreOl9LRWv9JkZULIniSwsODTl4nVcCqK-u-HOwed78fxSwBt35S~pNpCc1GU8kmlgg8g86RBwYW~u0wk7FCrTVFqDSsf9L6w9VObvv4FDd68xZ5cwpWWQ~dCOtaJN-shfGK~mMBPf2kwdgfeS07DPMxNIZdHaMK4Ea8JlRsDTt1h~IZTBc44GAH8Z4P58~1UoCtXlpv9jNeAkeQKvLWAz3A65XTInABIAMfNCp~Kv9EO-AJpxCVbheF~ndVMvxgZ2VW1kb2fj2fkX2SggMMi-r0bHTgn4q3iBaHxib0QJdBoNpTzw7Zhg3kFzbweXqVlEgKHxw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The role of Syk- and Src-family kinases in collagen- and CRP-mediated calcium increases in mouse megakaryocytes. Single mouse megakaryocytes from Balb/c bone marrow were microinjected with FURA-2, and the changes in [Ca2+]i were determined in response to (A) 100 μg/mL collagen or (B) 2 μg/mL CRP. The response to collagen (100 μg/mL) was then determined (C) after comicroinjection of a recombinant fusion protein containing the tandem SH2 domains of syk; (D) in syk-deficient megakaryocytes; (E) in the presence of the Src-family kinase inhibitor, PP1 (10 μmol/L for 3 minutes); or (F) in fyn-deficient megakaryocytes. Each trace is representative of 4 to 10 cells from a minimum of three different mice. In each case, [Ca2+]e was fixed at 200 μmol/L.
