Subcellular localization of MITF determined by immunocytochemistry. 293T cells were transfected with expression vector containing +-MITF (A), mi-MITF (B), or-MITF (C), orwh-MITF (D) cDNA. After 48 hours of transfection, cells were stained with anti-MITF antibody. (Original magnification × 4,000.)