Expression of EBV-specific genes, EBER1+2 and EBNA1-6, in different cell types after 3 weeks of cultivation with apoptotic bodies from EBV-carrying Namalwa line. (A) Phase contrast and (B) immunofluorescence staining showing coexpression of macrophage-specific marker CD68 (red) and EBNA (green) in the same cells. (C) shows the location of macrophage nuclei by Hoechst 33258 DNA staining that overlap with EBNA as shown in (D). Arrows mark the location of the nuclei that are located in the periphery of the cytoplasm of the macrophages. (E) Phase contrast and (F) fluorescence showing EBNA expression in bovine aortic endothelial cells. (G) Phase contrast and (H) bright field view of in situ hybridization analysis of EBER1 and 2 expression in HF cells. Arrows depict positive nuclei. Size bar = 20 μm.