Endothelial production of chemokines. HUVEC were left untreated, were infected with rAd.IκB- and/or stimulated with TNF- (100 U/mL) for 4 hours. (A) Quantification of soluble MCP-1 was performed by ELISA. Data are the mean ± SD of three separate experiments performed in duplicate. (B) Flow cytometric analysis was performed to quantitate the amount of surface-associated GRO-. Data are the mean ± SD of three separate experiments.