Flow cytometric analysis of endothelial adhesion molecule after infection with control vector rAd.GFP encoding GFP. (A) The expression of GFP in HUVEC was analyzed by flow cytometry after infection with rAd.GFP (solid line) or no treatment (dotted line) after 48 hours. (B and C) HUVEC infected with or without GFP control adenovirus were stimulated without (B) or with (C) TNF- (100 U/mL) for 4 hours and were stained with MoAb to ICAM-1 (solid or stippled line) or isotype control (dotted or dashed line). Shown are representative histograms.