Mithramycin strongly inhibits VEGF-induced TF activity. HUVEC were preincubated for 30 minutes with increasing concentrations of mithramycin (M) before stimulation with VEGF (A) or TNF- (B) for 4 hours. TF activity equivalents were measured in a one-stage clotting assay. (C) Mithramycin has no effect on TNF-–induced E-selectin expression measured by ELISA. X-axis: concentration of mithramycin. Each value is the mean ± SD of triplicate in one experiment representative of two performed with similar results.