American Society of Hematology

Fig. 10.

$Subcellular localization of tyrosine-phosphorylated proteins. (A) Western blots of cytosolic (S100, lanes 1, 3, and 5) and particulate fractions (P100, lanes 2, 4, and 6) fractionated from KG1a cells stimulated for 30 minutes with MOPC21 (control, lanes 1 and 2), anti-CD34 (4A1, lanes 3 and 4) or anti-CD43 (MEM59, lanes 5 and 6) as described in the legend to Fig 1, probed with anti-pTyr (top), reprobed with anti-CD34 (4A1), anti-CD43 (MEM59), anti-Syk, and anti-Lyn. Molecular size markers are indicated on the left (kD). (B) Western blots of equally adjusted amounts of Syk immunoprecipitates from cytosolic (S100, lanes 1, 3, and 5) or Triton X-100–solubilized membrane fractions (P100, lanes 2, 4, and 6) of KG1a cells stimulated as above, probed with anti-pTyr (top), and reprobed with anti-Syk (bottom). IgG (H), heavy chain of immunoglobulin G.$

Subcellular localization of tyrosine-phosphorylated proteins. (A) Western blots of cytosolic (S100, lanes 1, 3, and 5) and particulate fractions (P100, lanes 2, 4, and 6) fractionated from KG1a cells stimulated for 30 minutes with MOPC21 (control, lanes 1 and 2), anti-CD34 (4A1, lanes 3 and 4) or anti-CD43 (MEM59, lanes 5 and 6) as described in the legend to Fig 1, probed with anti-pTyr (top), reprobed with anti-CD34 (4A1), anti-CD43 (MEM59), anti-Syk, and anti-Lyn. Molecular size markers are indicated on the left (kD). (B) Western blots of equally adjusted amounts of Syk immunoprecipitates from cytosolic (S100, lanes 1, 3, and 5) or Triton X-100–solubilized membrane fractions (P100, lanes 2, 4, and 6) of KG1a cells stimulated as above, probed with anti-pTyr (top), and reprobed with anti-Syk (bottom). IgG (H), heavy chain of immunoglobulin G.

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