Fig. 4.
Capping of CD34 and CD43. KG1a cells were stimulated for 30 minutes with MOPC21 (control, a through c, p through r), anti-CD34 (My10, d through f; 4A1, g through i; QBEND10, j through l; 8G12, m through o), or anti-CD43 (MEM59, s through u) as described in the legend to Fig1. After fixation, cells were double-stained with anti-CD34 (green channel: a, d, g, j, and m) and phalloidin (red channel: b, e, h, k, and n) or with anti-CD43 (green channel: p and s) and phalloidin (red channel: q and t), and analyzed by confocal immunofluorescence microscopy. The right-hand panels show superimposed images of green and red channels. Bars, 5 μm.

Capping of CD34 and CD43. KG1a cells were stimulated for 30 minutes with MOPC21 (control, a through c, p through r), anti-CD34 (My10, d through f; 4A1, g through i; QBEND10, j through l; 8G12, m through o), or anti-CD43 (MEM59, s through u) as described in the legend to Fig1. After fixation, cells were double-stained with anti-CD34 (green channel: a, d, g, j, and m) and phalloidin (red channel: b, e, h, k, and n) or with anti-CD43 (green channel: p and s) and phalloidin (red channel: q and t), and analyzed by confocal immunofluorescence microscopy. The right-hand panels show superimposed images of green and red channels. Bars, 5 μm.

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