Fig. 3.
Coexpression of CD34 and CD43 on KG1a cells and stimulation with MoAbs to CD34 or CD43. (A) FACS analysis of KG1a cells stained with anti-CD34 (4A1) or anti-CD43 (MEM59). Solid lines represent staining with specific antibodies, and dotted lines represent staining with the secondary reagent alone. (B) Western blots of Triton X-100 lysates from KG1a cells, probed with anti-CD34 (4A1, lane 1) or anti-CD43 (MEM59, lane 2). (C) Western blots of equal amounts of Triton X-100 lysates from KG1a cells stimulated with anti-CD34 (4A1, lanes 2 through 7), anti-CD43 (MEM59, lanes 9 through 14) for the indicated times or incubated with MOPC21 (control IgG, lanes 1 and 8) for 30 minutes at 37°C, probed with anti-pTyr (4G10). Molecular size markers are indicated on the left (kD).

Coexpression of CD34 and CD43 on KG1a cells and stimulation with MoAbs to CD34 or CD43. (A) FACS analysis of KG1a cells stained with anti-CD34 (4A1) or anti-CD43 (MEM59). Solid lines represent staining with specific antibodies, and dotted lines represent staining with the secondary reagent alone. (B) Western blots of Triton X-100 lysates from KG1a cells, probed with anti-CD34 (4A1, lane 1) or anti-CD43 (MEM59, lane 2). (C) Western blots of equal amounts of Triton X-100 lysates from KG1a cells stimulated with anti-CD34 (4A1, lanes 2 through 7), anti-CD43 (MEM59, lanes 9 through 14) for the indicated times or incubated with MOPC21 (control IgG, lanes 1 and 8) for 30 minutes at 37°C, probed with anti-pTyr (4G10). Molecular size markers are indicated on the left (kD).

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