mRNA expression of pT and TCR β-chain by fractionated pooled primary colony cells. Total RNA was purified from a monocyte/macrophage cell line (Raw 264.7), thymocytes, and crude and fractionated pooled primary colony cells. Next, 0.3 μg to 0.5 μg of RNA was subjected to reverse transcription. One-tenth of the cDNA was then PCR-amplified for 45 cycles. Signals were visualized by using a DIG-conjugated probe and a DIG luminescent detection kit.