Sequence of the VH1 gene rearrangement of L1236 cells including part of the promoter region, the leader intron, and part of the constant region. The sequence obtained from L1236 cells is compared with germline sequences for the corresponding VH, JH, and Cγ4 genes (HV1F10,33JH4b,34 and Cγ4; Chai et al, unpublished data; Genbank Accession No. L23566) -, sequence identity; ., nucleotide deletion. Aminoacid changes are indicated by the germline amino acids given below the amino acid sequence of L1236. Codons are numbered according to Kabat et al.35 Corresponding amino acids are shown below each codon. The leader region, CDRI, CDRII, and CDRIII are indicated. Sequence already published7 is underlined. The sequence of Cγ4 and JH4B was obtained from cDNA. Therefore, no sequence is provided for the intron between JH4B and Cγ4. The RNA is correctly spliced. This sequence is available from the EMBL database under accession no. AJ005570.
