![Fig. 9. Effect of inhibition of PI3-kinase activity on the proliferation and apoptosis of HCD57 and HCD-SREI cells. (A) Cells cultured in the presence of 1 U EPO/mL (HCD57, ◊; HCD57-SREI, ⊖) or absence of EPO (HCD57-SREI, x) were treated with LY294002 at the indicated concentrations for 72 hours, and the viable cells were counted in the presence of trypan blue. Values are expressed as a percentage of the corresponding control (treated with vehicle dimethylsulfoxide [DMSO]). Data are means ± SE of at least triplicate measurements. (B) Cells were cultured in the presence of 1 U EPO/mL (HCD57) or absence of EPO (HCD57-SREI). HCD-57 (lanes 2 through 6) and HCD57-SREI (lanes 7 through 11) cells were treated with vehicle DMSO (lanes 3 and 8), LY294002 at indicated concentrations (lanes 4 through 6 and 9 through 11), or untreated (lanes 2 and 7) for 24 hours. The genomic DNA was isolated and analyzed for fragmentation of DNA characteristic of apoptosis as described in Materials and Methods.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/11/10.1182_blood.v93.11.3757/4/blod41134009bw.jpeg?Expires=1723714488&Signature=O-6jPFRyNjIBY4Qv2FzgQ62eWWkOmC6DBmsaZn0298VlvM-lRhXmRiZ0gE6rhbQ4buBKiMGY23uR6io0fBzLN9lFC~u4Z~h9AAQryqcg7I17pN3BcWV5V2bg5CXWcRARXg8Pco6UT7eaSpcB6I8cEyhf~Kh3PBvPlKN22lc6JROKZOr~fP1zOXvL93yFqNcHUa0i9peLTRnmnkeqMvlPN7rlQ9HzdIwKCDLBT1nu7pmvTo~wCUWH0LUkgHQpYyxSyPhYVT76AadptrY27F8ipNqcATDLko5aqFD-qOKzU5FF8sdzu-iFGiSwm0dJ9bNTeAWoMxeJXRUoplAAlpmXww__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of inhibition of PI3-kinase activity on the proliferation and apoptosis of HCD57 and HCD-SREI cells. (A) Cells cultured in the presence of 1 U EPO/mL (HCD57, ◊; HCD57-SREI, ⊖) or absence of EPO (HCD57-SREI, x) were treated with LY294002 at the indicated concentrations for 72 hours, and the viable cells were counted in the presence of trypan blue. Values are expressed as a percentage of the corresponding control (treated with vehicle dimethylsulfoxide [DMSO]). Data are means ± SE of at least triplicate measurements. (B) Cells were cultured in the presence of 1 U EPO/mL (HCD57) or absence of EPO (HCD57-SREI). HCD-57 (lanes 2 through 6) and HCD57-SREI (lanes 7 through 11) cells were treated with vehicle DMSO (lanes 3 and 8), LY294002 at indicated concentrations (lanes 4 through 6 and 9 through 11), or untreated (lanes 2 and 7) for 24 hours. The genomic DNA was isolated and analyzed for fragmentation of DNA characteristic of apoptosis as described in Materials and Methods.
