Jaspamide induces differentiation in HL-60 cells. HL-60 cells were grown for 48 hours with (A) 0.01% DMSO, or (B) 10⁻⁷ mol/L jaspamide. Cell differentiation was determined on Wright-Giemsa–stained cytocentrifuge preparations. (C) FACS analysis and overlay histograms of fluorescence intensity showing the upregulation of the differentiation antigen CD16 in HL-60 cells after exposure of the cells for 48 hours to 10⁻⁷ mol/L jaspamide (thick line) or 0.01% DMSO (broken thin line). An irrelevant antibody was used as background control (dotted line). M₁line defines the positive intensities range (A, B: original magnification × 475).