Fig. 2.
The effect of preincubation of R3 anti-uPAR antibody on stimulation of plasmin generation by U937 cells and Nalm6 cells. Data show the activation rate with 100 nmol/L glu-plasminogen and cell concentrations of 2.83 ± 0.4 × 106 U937 cells/mL and 1.25 ± 0.27 × 106 Nalm6 cells/mL. Before activity determinations, approximately 2 × 107 cells/mL were preincubated with 225 nmol/L R3 antibody (columns labeled R3) or 1 nmol/L uPA (columns labeled uPA). R3/uPA denotes preincubation with R3 followed by incubation with 1 nmol/L uPA. Columns labeled uPA* show activation rates where 15 pmol/L uPA was added to the final reaction mixture containing cells and 100 nmol/L glu-plasminogen. Activation rates are shown as mean ± SE, n = 3.
