American Society of Hematology

Fig. 4.

Representative Southern blot analysis of individual NOD/SCID mice transplanted with 1 × 104, 2 × 104, and 3.5 × 104 unmanipulated CD34+ CB cells and with week 4 expanded cells (deriving from 1 × 104 initial CD34+ cells). DNA was extracted from the murine BM at week 8 after transplant and hybridized with a human chromosome 17–specific -satellite probe.

Representative Southern blot analysis of individual NOD/SCID mice transplanted with 1 × 10⁴, 2 × 10⁴, and 3.5 × 10⁴ unmanipulated CD34⁺ CB cells and with week 4 expanded cells (deriving from 1 × 10⁴ initial CD34⁺ cells). DNA was extracted from the murine BM at week 8 after transplant and hybridized with a human chromosome 17–specific -satellite probe.

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