(A) Representative fluorescence-activated cell sorting (FACS) profiles of marrow cells from NOD/SCID mice transplanted 8 weeks previously with unmanipulated CD34+ CB cells. (a) Negative control: a nonengrafted mouse (transplanted with 2 × 105 irradiated CD34− CB carrier cells). (Middle) A mouse transplanted with 1 × 105CD34+ CB cells. (Bottom) A mouse transplanted with 2 × 104 CD34+ cells. CD45/CD34 and CD45/CD19 analysis was performed on total BM cells. (B) Multilineage engraftment in the BM of a representative mouse transplanted with 1 × 105 unmanipulated CD34+ CB cells. Analysis of lineage markers (CD45/CD34, CD19, CD41, and CD13/CD33) was performed on cells comprised within the CD45 gate. Analysis of GpA/CD71-positive cells was performed on total BM cells.
