American Society of Hematology

Fig. 1.

Representative DNA analysis of human cell engraftment in the BM of NOD/SCID mice transplanted with 1 × 104, 2 × 104, and 3.5 × 104 unmanipulated CD34+ CB cells. Cells were injected together with preirradiated 2 × 105 CD34− CB cells as carrier cells. Human DNA was assessed by Southern analysis using a human chromosome 17-specific -satellite probe. Human:mouse controls are given as percent human DNA.

Representative DNA analysis of human cell engraftment in the BM of NOD/SCID mice transplanted with 1 × 10⁴, 2 × 10⁴, and 3.5 × 10⁴ unmanipulated CD34⁺ CB cells. Cells were injected together with preirradiated 2 × 10⁵ CD34⁻ CB cells as carrier cells. Human DNA was assessed by Southern analysis using a human chromosome 17-specific -satellite probe. Human:mouse controls are given as percent human DNA.

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