Fig. 4.
![Fig. 4. FPR phosphorylation and MAP kinase activation induced by T20/DP178. FPR phosphorylation was measured by incubating [32P]-orthophosphate–labeled ETFR cells with medium alone or T20/DP178 at 37°C for 15 minutes, and the same amount of cell lysate for each treatment was immunoprecipitated with an antiepitope (anti-HA) antibody (A). MAPK activation was examined in human monocytes by treatment of the cells with different concentrations of T20/DP178 at various time intervals and immunoprecipitation of the cell lysates with an anti-p38 MAPK antibody, followed by immunoblotting with a mouse antiphosphotyrosine antibody (B). In time-course experiments of MAPK activation, 10 μmol/L T20/DP178 was used.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/11/10.1182_blood.v93.11.3885/4/blod41119004bw.jpeg?Expires=1724944469&Signature=4GM-Ge6dYU7IgWKVlNh8Hb0Yzb9WjQtSANpb4jnwX0WLj9NKVLqDaXlZpIgxSg~u2K4ApBtx6~YgMjmX~TyfppIMav7mAa6V82eGfqaoTYJuj71pBpLufCj1~BxTGsq3Te1LoWBvlIohwOYxgCeSzDnpz7AwOe1816Z~13b~Y1rsg8tHPhIDcvR2K59hBUro3Sf7Xi0ystDNbk06HtoxKhnhRRzm~IndfEQpOD6bV04rA~WsOq96-pdfsPhqmi-UmLmUy9gFhOiSyUjvagT6TFAKGC09Um5L9ya75Kiprqun~MTBrwodCBIgZg9dOjshgHuFQVKrrN~6O8Aj3Q36TQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
FPR phosphorylation and MAP kinase activation induced by T20/DP178. FPR phosphorylation was measured by incubating [32P]-orthophosphate–labeled ETFR cells with medium alone or T20/DP178 at 37°C for 15 minutes, and the same amount of cell lysate for each treatment was immunoprecipitated with an antiepitope (anti-HA) antibody (A). MAPK activation was examined in human monocytes by treatment of the cells with different concentrations of T20/DP178 at various time intervals and immunoprecipitation of the cell lysates with an anti-p38 MAPK antibody, followed by immunoblotting with a mouse antiphosphotyrosine antibody (B). In time-course experiments of MAPK activation, 10 μmol/L T20/DP178 was used.
