Multiple alignments of inferred amino acid sequences from ORF26 nucleotides sequences of HHV-8 detected in tissue samples from 6 patients with POEMS syndrome, 1 HIV-infected patient with Kaposi’s sarcoma and multicentric Castleman’s disease (KS/MCD), 8 healthy African blood donors (ABD), and 14 sequences previously published, including the 12 precedently used for phylogenetic analysis (KSHV AIDS, ST1 AIDS KS, ST2 AIDS KS, ST3 AIDS KS, C282 AIDS KS, ASM70 Lung KS, AKS1 AIDS KS, 431 KAP Endemic KS, EKS1 non-AIDS KS, BCBL-1, BCBL2, and BCBLR AIDS Lym), 1 sequence from an HIV⁻ patient with monocentric Castleman’s disease (case 9),43 and sequences of HHV-8 detected in three cases of reactive lymphadenopathy (cases 6, 10, and 16).45 By comparison with the 111 amino acid consensus sequence of ORF26 deduced from these latter sequences, the base changes of HHV-8 in patients with POEMS syndrome at positions 1032 and 1033 encode a lysine to isoleucine substitution in codon 134, and the base change at position 1132 encodes an aspartate to glycine substitution in codon 167. The positional nomenclature used for ORF26 amino acid sequences follows that of Chang et al.3 Hyphens (-) indicate sequence homology, and dots (·) indicate gaps introduced for optimal alignment. Single-letter abbreviations for the amino acid residues are: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr. BM, bone marrow; KS, Kaposi’s sarcoma; Lym, lymphoma; LN, lymph node; Myel, myeloma.