Fig. 5.
Fig. 5. Size exclusion chromatography of glycosaminoglycan chains obtained after β-elimination of proteoglycans from the supernatant (A) and cell extract (B) of the immature erythroid progenitor cell line HEL. Proteoglycans from the supernatant (A) and cell extract (B) of HEL cells were treated first either by heparinase/heparitinase or chondroitinase AC/ABC and thereafter submitted to β-elimination. Size exclusion chromatography was performed on a TSK 4000 column. Elution profiles were monitored by liquid scintillation counting.

Size exclusion chromatography of glycosaminoglycan chains obtained after β-elimination of proteoglycans from the supernatant (A) and cell extract (B) of the immature erythroid progenitor cell line HEL. Proteoglycans from the supernatant (A) and cell extract (B) of HEL cells were treated first either by heparinase/heparitinase or chondroitinase AC/ABC and thereafter submitted to β-elimination. Size exclusion chromatography was performed on a TSK 4000 column. Elution profiles were monitored by liquid scintillation counting.

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