Fig. 7.
Membrane-bound IL-15 is biologically active. Human purified CD3+ T cells were incubated with medium, IL-15 (10 ng/mL), IFN-γ–treated and mitomycin C-treated monocytes, or the latter plus anti–IL-15, anti–IL-2, or anti–IL-2Rβ antibodies (10 μg/mL), all in presence of ConA (10 μg/mL). Proliferative activity was assessed by a (3H) thymidine incorporation assay. One representative experiment of three that produced similar results is shown. Significance was analyzed by comparison between antibody-treated and untreated cells with Fisher’s Least-test. *Significant induction by treatment (P < .05). §Significant inhibition by treatment (P < .05).
