Fig. 3.
Fig. 3. HAF-1 and PU.1 complexes are disrupted/supershifted by antisera raised against ets family members. EMSA was performed as described in Materials and Methods. Elf-1 or PU.1 antisera and corresponding blocking peptides were added where indicated. Antisera were added after preincubation with or without 0.4 μg of blocking peptide. Similar to Fig 2, the bottom left-hand image is a shorter exposure of the upper autoradiogram to permit resolution of the HAF-1/CP1 doublet.

HAF-1 and PU.1 complexes are disrupted/supershifted by antisera raised against ets family members. EMSA was performed as described in Materials and Methods. Elf-1 or PU.1 antisera and corresponding blocking peptides were added where indicated. Antisera were added after preincubation with or without 0.4 μg of blocking peptide. Similar to Fig 2, the bottom left-hand image is a shorter exposure of the upper autoradiogram to permit resolution of the HAF-1/CP1 doublet.

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