Fig. 3.
![Fig. 3. Semiquantitative RT-PCR analysis of GPIb, GPIbβ, PF4, c-mpl, and p45 NF-E2 mRNA transcripts from primary megakaryocytes derived from normal (WT) and neo▵HS (MUT) CFU-Mk. PCR reactions were performed with tracer [32P]dCTP for the indicated number of cycles before analysis by PAGE. HPRT signal was used to normalize the input cDNA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/9/10.1182_blood.v93.9.2867/4/blod40924003w.jpeg?Expires=1724279570&Signature=Q10ApAltSMQidI8Gta5fCGsmQrc0EYR~RPYF06tkoeOmxTVvha6oM9r~OHj7yiEaj5k9wu0oWuGzJqPEmEVMJKLutmmERNqJQ0awnP70yxUNiz4Pfeh8u8R8tD6V4NFV0nwi5svsticVx4NLhyFDT3l0E6yMd607TC0SQ-B1o9yWFuRm2xX50xV3D7IAusCp2BUfKz-oSAxKLQHsd5cSqJOWge5GjWU7pPgdyE-kTa~gnldJAdxotDaB6CBtAuWsf2~0XLSJl43-7BvAKn2CjzlesKBPqZ4In5vQaPzOYcimT1Xd9aqt9Prqk8VATrGHb4oOYvFtIu13~iC6uuFgnQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Semiquantitative RT-PCR analysis of GPIb, GPIbβ, PF4, c-mpl, and p45 NF-E2 mRNA transcripts from primary megakaryocytes derived from normal (WT) and neo▵HS (MUT) CFU-Mk. PCR reactions were performed with tracer [32P]dCTP for the indicated number of cycles before analysis by PAGE. HPRT signal was used to normalize the input cDNA.
