Antisense ODN to HOX A5 decrease levels of HOX A5 mRNA. K562 cells transduced to overexpress HOX A5 are treated with 15 μmol/L of antisense, sense, nonsense (scrambled antisense), or no ODN for 24 hours. Lanes 1 through 4 (+RT) contain cDNAs generated from each treatment and were subjected to PCR amplification with HOX A5- and GAPDH-specific primers. Lanes 6 through 10 (−RT) contain HOX A5 and GAPDH PCR products of mock cDNA reactions lacking reverse transcriptase. Ethidium bromide-stained gels were digitally scanned, and relative intensities of HOX A5 product bands compared with their corresponding GAPDH bands are denoted below the lanes.