Fig. 9.
Fig. 9. Effect of caspase-3 inhibition on expression of Bcl-Xl. PMN were treated for 1 hour at 37°C with 200 μmol/L of the caspase-3 inhibitor Z-DEVD-FMK before stimulation for indicated times with 300 U/mL TNF-, 300 U/mL GM-CSF, or left untreated (control), respectively. Whole-cell lysates were subjected to SDS-PAGE and Western blot was performed using the anti–Bcl-X Ab and a peroxidase-conjugated secondary antibody. Results are representative of three independent experiments.

Effect of caspase-3 inhibition on expression of Bcl-Xl. PMN were treated for 1 hour at 37°C with 200 μmol/L of the caspase-3 inhibitor Z-DEVD-FMK before stimulation for indicated times with 300 U/mL TNF-, 300 U/mL GM-CSF, or left untreated (control), respectively. Whole-cell lysates were subjected to SDS-PAGE and Western blot was performed using the anti–Bcl-X Ab and a peroxidase-conjugated secondary antibody. Results are representative of three independent experiments.

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