Expression of transcription factors GATA-1, GATA-2, and p45 NF-E2 in CB CD34⁺Lin⁻ cells induced to unilineage erythroid differentiation. Transcription factor expression was evaluated by immunohistochemistry (immunogold staining with silverenhancement method) using specific MoAbs (original magnification × 400 and × 1,000). Arrows indicate nuclear staining of selected cells. Control staining was performed with irrelevant, isotype-matched rat or mouse MoAbs as well as with irrelevant rabit antibody.