Analysis of ¹²⁵I-IL-8 binding to HMC-1 cells. (A) A representative equilibrium-binding analysis is shown, and Scatchard plot transformation of the binding data is presented in the insert. (B) Concentration-dependent displacement of¹²⁵I-IL-8 binding to HMC-1 cells by IL-8 (•), GRO (▴), IP-10 (▪), and MIG (▾). HMC-1 cells (5 × 10⁵ cells in each tube) were incubated with¹²⁵I-IL-8 (0.2 nmol/L) in the presence or absence of increasing concentrations of unlabeled ligand for 60 minutes at 4°C.