Fig. 1.
![Fig. 1. Epo but not osmotic shock or heat shock lead to activation of HPK1 kinase activity. (A) In vitro HPK1 autophosphorylation in Epo-dependent FD-EPO cells (A) and in Epo-responsive SKT6 cells (B). HPK1 was immunoprecipitated using a HPK1-specific antibody after Epo stimulation at the indicated time points (lanes 1 through 6) or after 0.35 mol/L NaCl treatment for 30 minutes (lane 7) or heat shock at 42°C for 30 minutes (lane 8). In vitro kinase assays were performed in the presence of [γ-32P]ATP. The arrow indicates the autophosphorylated HPK1, and the band below is nonspecific.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/10/10.1182_blood.v93.10.3347.410k06_3347_3354/5/blod41006001w.jpeg?Expires=1724247526&Signature=wSANYej26TbRlfW66aHg6xBLoGwBql0OG6gO9Gxy3vC1SXgRMgvTc2888jXWRdCfM2dWSPc~Ac-emxi6bbtrzmIhyMX4oE49HfC75vqUmEFRcFEtoOZdQfSzT-K26UhvV~8tsc9FeiFAwwLfjmvB4UEGZ21YJC7qn7EvfpQpDs0-CfvnzoQxc1n1I2NEqUbdIhloE6i0AMlOoBdYsuuKnXm0A198kzOddMHzNGUcHPYveDlvqmskYd-3kloF1nbkiWPPCjfaI4Qdk2rqThoOPJ1iKyF5o6Eb~D1RwBs0O29ETWelcbiq6fCZpCXiR~5nxB1w06yeXgWNZl2WoEtfGA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Epo but not osmotic shock or heat shock lead to activation of HPK1 kinase activity. (A) In vitro HPK1 autophosphorylation in Epo-dependent FD-EPO cells (A) and in Epo-responsive SKT6 cells (B). HPK1 was immunoprecipitated using a HPK1-specific antibody after Epo stimulation at the indicated time points (lanes 1 through 6) or after 0.35 mol/L NaCl treatment for 30 minutes (lane 7) or heat shock at 42°C for 30 minutes (lane 8). In vitro kinase assays were performed in the presence of [γ-32P]ATP. The arrow indicates the autophosphorylated HPK1, and the band below is nonspecific.
