Effect of anti-integrin antibody on CD43-enhanced MO7e and cord blood CD34⁺ cell adhesion to fibronectin. (A) MO7e cells were treated with or without anti-CD43 MoAb (clone DF-T1) and goat antimouse IgG. CD43-stimulated cells were incubated with 10 μg/mL control IgG or anti-integrin antibody for an additional 20 minutes at 4°C and subjected to adhesion assay. Data represent the mean ± SE of triplicate assays for one of two reproducible experiments. *P < .0001, **P < .001 versus CD43-stimulated adhesion without additional antibodies. (B) Sorted CD34⁺ cells were treated with 20 μg/mL control IgG or CD43 (clone DF-T1) followed by goat antimouse IgG. CD43-stimulated cells were incubated with 10 μg/mL anti-integrin antibody for an additional 20 minutes at 4°C and subjected to adhesion assay. Data represent the mean ± SE from three separate experiments with triplicate samples for each group in every experiment. *P < .01, **P < .05 versus CD43-stimulated adhesion without additional antibodies.