Fig. 4.
Fig. 4. Expression of ▵LNGFR in liquid cultures of control (C), SFCM-transduced, and GATA-SFCM–transduced CD34+ cells. Cultures were analyzed 48 hours after transduction for expression of ▵LNGFR and CD34 (day 6) and 2 days after induction of differentiation for expression of ▵LNGFR and the lineage-specific markers GpA and CD13 (day 8). CD34+, GpA+, and CD13+ cells are shown in blue, red, and green colors, respectively. Values indicate the percentage of ▵LNGFR+cells in the CD34+, GpA+, or CD13+ fractions (boxed). Cells were stained with FITC-conjugated anti-LNGFR (X axis) and PE-conjugated anti-CD34, GpA, and CD13 (Y axis) antibodies.

Expression of ▵LNGFR in liquid cultures of control (C), SFCM-transduced, and GATA-SFCM–transduced CD34+ cells. Cultures were analyzed 48 hours after transduction for expression of ▵LNGFR and CD34 (day 6) and 2 days after induction of differentiation for expression of ▵LNGFR and the lineage-specific markers GpA and CD13 (day 8). CD34+, GpA+, and CD13+ cells are shown in blue, red, and green colors, respectively. Values indicate the percentage of ▵LNGFR+cells in the CD34+, GpA+, or CD13+ fractions (boxed). Cells were stained with FITC-conjugated anti-LNGFR (X axis) and PE-conjugated anti-CD34, GpA, and CD13 (Y axis) antibodies.

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