Multiparametric FACS analysis of human cord blood CD34⁺ cells 48 hours after transduction with SFCM and GATA-SFCM vectors. The forward (FSC) and side (SSC) scatter plot is in the upper-left panel. Expression of ▵LNGFR (lower panels) was analyzed in the low-SSC, CD34⁺, CD38^low gated cell population (boxed in the upper-right panel). The percentage of ▵LNGFR⁺ cells (boxed) is indicated in the lower panels. Cells were stained with FITC-conjugated anti-LNGFR, TC-conjugated anti-CD38 (X axis) and PE-conjugated anti-CD34 (Y axis) antibodies. C, control, untransduced cells.