SSCP analysis of plasminogen gene exon 5 in patient no. 5 and his healthy parents. Radiolabeled single-stranded PCR products of plasminogen exon 5 were electrophoresed in a nondenaturing 5% polyacrylamide gel as described in Materials and Methods. Lane 1, healthy control (C); lane 2, father of patient no. 5; lane 3, mother of patient no. 5; lane 4, patient no. 5. dsDNA, double-stranded DNA.