Fig. 9.
Fig. 9. Distribution of CD11b on the neutrophil surface after cross-linking of CD66b. Upper left panel, neutrophil exposed to FITC-conjugated anti-CD11b MoAb; upper right panel, neutrophil exposed to anti-CD66b MoAb before incubation with a second step goat-F(ab2’) fragment against mouse MoAbs to cross-link CD66b and labeled with FITC-conjugated anti-CD11b MoAb; lower left panel, neutrophil treated as in the upper right panel, but in the presence of 100 mmol/L D-mannose; lower right panel, neutrophil exposed to anti-CD66b MoAb before incubation with a second step goat-F(ab2′) fragment against mouse MoAbs to cross-link CD66b and labeled with FITC-conjugated anti-CD10 MoAb.

Distribution of CD11b on the neutrophil surface after cross-linking of CD66b. Upper left panel, neutrophil exposed to FITC-conjugated anti-CD11b MoAb; upper right panel, neutrophil exposed to anti-CD66b MoAb before incubation with a second step goat-F(ab2’) fragment against mouse MoAbs to cross-link CD66b and labeled with FITC-conjugated anti-CD11b MoAb; lower left panel, neutrophil treated as in the upper right panel, but in the presence of 100 mmol/L D-mannose; lower right panel, neutrophil exposed to anti-CD66b MoAb before incubation with a second step goat-F(ab2′) fragment against mouse MoAbs to cross-link CD66b and labeled with FITC-conjugated anti-CD10 MoAb.

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