Neutrophil-mediated cytolysis in the absence or presence of 10 μg/mL Lym-1 and/or 1 ng/mL GM-CSF.51Cr-labeled Raji cells were at 2 × 104. The neutrophil:Raji cell ratio was 20:1. The incubation time was 14 hours. In the absence of Lym-1 and GM-CSF (Nil), neutrophil-mediated lysis was 0.25 ± 0.77 (mean ± 1 SD, n = 21) with a median of 0.00 (confidence interval 95%, −0.09 to 0.60). In the presence of GM-CSF, the lysis was 1.97 ± 2.28 (mean ± 1 SD, n = 31) with a median of 0.80 (confidence interval 95%, 1.14 to 2.81). In the presence of Lym-1, the lysis was 5.08 ± 7.5 (mean ± 1 SD, n = 82) with a median of 2.2 (confidence interval 95%, 3.43 to 6.73). In the presence of both GM-CSF and Lym-1, the lysis was 29.14 ± 16.29 (mean ± 1 SD, n = 82) with a median of 27.20 (confidence interval 95%, 25.55 to 32.73). Neutrophil-mediated lysis in the presence of Lym-1 versus that observed in the absence (Nil) was P < .01. The lysis in the presence of GM-CSF versus that observed in the absence (Nil) was P > .05. Finally, the lysis in the presence of both Lym-1 and GM-CSF versus that in the absence (Nil) or that in the presence of Lym-1 or GM-CSF alone was always P < .001.
