Adhesion of uPAR-transfected BAF-3 cells on FBG. (A) Adhesion of BAF-3 cells, transfected with uPAR in the sense or antisense orientation, was tested on FBG in response to Mn²⁺ (50 μmol/L), Zn²⁺ (50 μmol/L), or PMA (20 ng/mL), respectively, in the absence (▩) or presence of 10 μg/mL of MoAb Game 46 (▨) against mouse β₂-integrins. (B) A similar adhesion assay was performed, except that either HEPES-buffered saline without (▩) or with Ca²⁺ and Mg²⁺ (▨) were used as the adhesion buffer instead of RPMI 1640. Data represent the mean ± SEM (n = 3) of a typical experiment. Similar results were obtained in three separate experiments.