The antioxidant PDTC reduces intracellular levels of ROS and cell growth in MO7e cells. (A) MO7e cells were stimulated for 18 hours with 10 ng/mL GM-CSF or 10 ng/mL IL-3 before treatment for 3 hours with 25 μmol/L PDTC (dotted line) or left untreated (straight line) as indicated. The autofluorescence of these cells (top panel) or the relative levels of ROS using 2’, 7’-dichloro-fluorescin-diacetate (bottom panel) were measured. (B) MO7e cells were treated with the indicated doses PDTC, NAC, and 2-mercaptoethanol (2-ME) for 72 hours, and cell growth was measured by trypan blue exclusion. The error bars indicate the standard error of the mean (SEM) (n = 4).