Fig. 1.
Competitive binding of native and carbohydrate remodeled placental-derived and recombinant glucocerebrosidase to rat alveolar macrophages. Rat alveolar macrophages were incubated with125I-mannosylated BSA (0.25 μg/mL) at 4°C in the presence of increasing concentrations of rGC (⧫), pGC (x ), rGCR (▪), and pGCR (▴). Binding of the radiolabeled ligand to the cells was determined as described in Materials and Methods.
