Contribution of TNF-–, FasL-, or perforin-mediated cytotoxicity to the GVL effect. GVL effect was evaluated in BDF1 mice that were inoculated IV with 1 × 104 L1210 or 1 × 105 P815 cells 2 days before the BMT as described in Fig1B. (A) Administration of anti–TNF- MoAb to the recipients of B6 BMS resulted in early death within 16 days. Six of 10 gld BMS recipients survived more than 80 days, whereas all B6 BMS or PKO BMS recipients died within 51 days. (B) Similar results were also observed by using another tumor P815 cells. Administration of anti–TNF- MoAb resulted in early death within 24 days. Six of 8 gld BMS recipients survived more than 80 days, whereas all B6 BMS or PKO BMS recipients died within 47 days. Data represent the results of three similar experiments.
