Phenotype of BV15⁺ T-cell clone with CDR3 sequence of DLTSGP in patient no. 5. cDNA derived from CD4⁺ and CD8⁺ T cells of BM from patient no. 5 was amplified using a primer specific to BV15 coupled with a BC primer, and the amplified products were submitted to primer extension using the fluorescent clonotypic primer containing the DLTSGP motif. Amplified BV15 cDNA was analyzed for size with the gene scan program.