Fig. 1.
Fig. 1. CDR3 size distribution of TCR BV derived from BM T cells of AA patients who obtained unmaintained remission early after CyA therapy. cDNA that was amplified using primers specific for 24 different BV subfamilies and coupled with a fluorescent BC primer was analyzed for size with the gene scan program. N1 and N2, normals no. 1 and 2 possessing HLA-DRB1*1501; P1-P4, patients no. 1-4. Most BV subfamilies of the patients and normal controls displayed a bell-shaped size pattern with >5 peaks, indicating the predominance of polyclonal T cells.

CDR3 size distribution of TCR BV derived from BM T cells of AA patients who obtained unmaintained remission early after CyA therapy. cDNA that was amplified using primers specific for 24 different BV subfamilies and coupled with a fluorescent BC primer was analyzed for size with the gene scan program. N1 and N2, normals no. 1 and 2 possessing HLA-DRB1*1501; P1-P4, patients no. 1-4. Most BV subfamilies of the patients and normal controls displayed a bell-shaped size pattern with >5 peaks, indicating the predominance of polyclonal T cells.

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