Id-specific CTL activity of patient 1. Bulk PBMNC obtained 4 weeks after the second Id-pulsed DC vaccination were stimulated for 12 days with either autologous Id or irrelevant isotype matched control Ig. Stimulated effector cells (E) were coincubated with autologous 51Cr-labeled fibroblasts (FB) targets (T) expressing either Id or a bacterial control gene (LacZ) via Ad transduction. Various effector to target (E:T) ratios were used and specific lysis of FB targets in a 4-hour chromium-release assay was calculated on the basis of triplicate wells. Error bars denote one standard deviation. Id-stimulated effector cells killed significantly more (P = .05) IdAd expressing targets than control targets at an E to T ratio of 100:1, 33:1, and 11:1 (Mann-Whitney U test).
